Ph.D.

　University Laboratory of Physiology, Oxford University, UK

M.D.

　Department of Medicine, Kaohsiung Medical University, Taiwan

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1. Cell Volume Regulation in Epithelial Cancers

Homeostasis of cell volume is a fundamental cellular property . Alterations of cell volume and volume regulatory mechanisms participate in a wide variety of physiological and pathological cellular functions. Our research work focuses on the important role of cell volume regulation in epithelial cancers. Three parts of studies are included:

(1)Cellular and molecular mechanisms

We are using different types of cells representing the various stages of carcinogenesis to study the role of KCC isoforms in tumor cellular and molecular biology.

(2) KCC knockout mouse model

We are using the KO animal model to establish the important role of KCC 3 in angiogenesis and tumor immunity.

(3)Translational research

This translational study aims to identify novel gynecological cancer biomarkers and potential therapeutic targets through molecular analysis of cell volume regulator genes/proteins. (i.e. KCC3, KCC4 and NHE1)

Research Model of Cell Volume Regulation in Epithelial Cancers

2. The Effects of Soft Substratum on Epithelial Cancinogenesis

We previously demonstrated that soft substrate of collagen gel induced apoptosis in polarized cells, but not in transformed cells or fibroblasts. Ca 2+ signaling plays an important role in the regulation of cell response to different substrate rigidities. There is now convincing evidence that the alteration of Ca 2+ homeostasis is associated with cell survival and death. A Ca 2+ -based theory of carcinogenesis has been proposed. For example, alteration of Ca 2+ homeostasis has been demonstrated to initiate oncogenesis, which leads to cell transformation. Whether there exists any relationship between substratum properties and carcinogenesis is our interest.

3. The Role of Epidermal Growth Factor Induced Epithelial-Mesenchymal Transition in Cervical Cancer

The process of epithelial-mesenchymal transition (EMT) was highly associated with cervical cancer invasion and EGF played an important role in cervical EMT. We are interested in molecular mechanisms by which EGF downregulated E-cadherin and beta-catenin expression. Moreover, blockade of integrin alpha5 beta1 signaling significantly modulated EGF-mediated EMT. Unraveling the process of EMT could provide valuable information on cervical cancer malignancy.

1. Huang YT, Hsu YT, Wu PY, Yeh YM, Lin PC, Hsu KF, Shen MR* (corresponding author) (2024). Tight junction protein cingulin variant is associated with cancer susceptibility by overexpressed IQGAP1 and Rac1-dependent epithelial-mesenchymal transition. Journal of Experimental & Clinical Cancer Research, 43(1):65.

2. Hsu YT, Chen LH, Liu YH, Chu SK, Chen TY, Tsai KJ, Shen MR*, Liu W* (corresponding author) (2023). Electrical sympathetic neuromodulation protects bone marrow niche and drives hematopoietic regeneration during chemotherapy. Small Methods, 7(6):

3. Lin PC, Tsai YS, Yeh YM, Shen MR*. (corresponding author) (2022) Cutting-edge AI technologies meet precision medicine to improve cancer care. Biomolecules, 12(8):1133.

4. Lai CH, Li KW, Hu FW, Su PF, Hsu IL, Huang MH, Huang YT, Liu PY, Shen MR*. (corresponding author) (2022) Integration of an intensive care unit visualization dashboard (i-Dashboard) as a platform to facilitate multidisciplinary rounds: cluster-randomized controlled trial. Journal of Medical Internet Research, 24(5):e35981.

5. Chen YF, Wu CH, Chen LH, Lee HW, Lee JC, Yeh TK, Chang JY*, Chou MC, Wu HL, Lai YP, Song JS, Yeh KC, Chen CT, Lee CJ, Shia KS*, Shen MR*. (corresponding author) (2022) Discovery of potential neuroprotective agents against paclitaxel-induced peripheral neuropathy. Journal of Medicinal Chemistry, 65(6):4767-4782.

6. Chan RH, Chen PC, Yeh YM, Lin BW, Yang KD, Shen MR*, Lin PC*. (corresponding author) (2022) The expression quantitative trait loci in immune response genes impact the characteristics and survival of colorectal cancer. Diagnostics, 12(2):315.

7. Chen YF, Shen MR*. (corresponding author) (2022) The important role of ion transport system in cervical cancer. International Journal of Molecular Sciences, 23(1):333.

8. Yeh YM, Lin PC, Su WC, Shen MR*. (corresponding author) (2021) CD40 pathway and IL-2 expression mediate the differential outcome of colorectal cancer patients with different CSF1R1085 genotypes. International Journal of Molecular Sciences, 22(22):12565.

9. Huang YT, Hsu YT, Chen YF, Shen MR*. (corresponding author) (2021) Super-resolution microscopy reveals that STIM1 trafficking depends on microtubule dynamics. Frontiers in Physiology, 12:762387.

10. Lin PC, Yeh YM, Hsu HP, Chan RH, Lin BW, Chen PC, Pan CC, Hsu KF, Hsiao JR, Shan YS, Shen MR*. (corresponding author) (2021) Comprehensively exploring the mutational landscape and patterns of genomic evolution in hypermutated cancers. Cancers, 13(17):4317.

11. Yeh YM, Wu PY, Lin PC, Su PF, Hsu YT, Hsu KF*, Shen MR*. (corresponding author) (2021) MET mutation is a potential therapeutic target for advanced endometrial cancer. Cancers, 13(16):4231.

12. Lin PC, Yeh YM, Chan RH, Lin BW, Chen PC, Pan CC, Shen MR*. (corresponding author) (2021) Sequential and co-occurring DNA damage response genetic mutations impact survival in stage III colorectal cancer patients receiving adjuvant oxaliplatin-based chemotherapy. BMC Cancer, 21(1):217.

13. Yeh YM, Lin PC, Lee CT, Chen SH, Lin BW, Lin SC, Chen PC, Chan RH, Shen MR*. (corresponding author) (2020) Treatment monitoring of colorectal cancer by integrated analysis of plasma concentration and sequencing of circulating tumor DNA. Molecular Cancer, 19:150.

14. Lin PC, Yeh YM, Lin BW, Lin SC, Chen PC, Shen MR*. (corresponding author) (2020) Intratumor heterogeneity of MYO18A and FBXW7 variants impact the clinical outcome of stage III colorectal cancer. Frontiers in Oncology, 10:588557.

15. Chen LH, Yeh YM, Chen YF, Hsu YH, Wang HH, Lin PC, Chang LY, Lin CK, Chang MS*, Shen MR*. (corresponding author) (2020) Targeting interleukin-20 alleviates paclitaxel-induced peripheral neuropathy. Pain, 161(6):1237-1254.

16. Liu PY, Tsai YS, Chen PL, Tsai HP, Hsu LW, Wang CS, Lee NY, Huang MS, Wu YC, Ko WC, Yang YC, Chiang JH, Shen MR*. (corresponding author) (2020) Application of an artificial intelligence trilogy to accelerate processing of suspected patients with SARS-CoV-2 at a Smart quarantine station: observational study. Journal of Medical Internet Research, 22(10):e19878.

17. Chen YF, Lin PC, Yeh YM, Chen LH, Shen MR*. (corresponding author) (2019) Store-operated Ca²⁺ entry in tumor progression: from molecular mechanisms to clinical implications. Cancers, 11(7):899.

18. Lin PC, Yeh YM, Wu PY, Hsu KF, Chang JY, Shen MR*. (corresponding author) (2019) Germline susceptibility variants impact clinical outcome and therapeutic strategies for stage III colorectal cancer. Scientific Reports, 9(1):3931.

19. Chen YF, Chen LH, Shen MR*. (corresponding author) (2019) The distinct role of STIM1 and STIM2 in the regulation of store‐operated Ca²⁺ entry and cellular function. Journal of Cellular Physiology, 234(6):8727-8739.

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Research NCKU ! (Open Link)